Studies on Human Haptoglobins. IV

Immunogenetics of Human Haptoglobins

A B S T R A C T Using a radioimmunoassay method in conjunction with double diffusion studies, we characterized the antigenic determinants of the three Hp Bellevue phenotypes. An antigenic model based on these data indicates that each phenotype comprises a heterogeneous group of proteins with properties depending on their content of normal (hp,8) and of mutant (hp,8Bellevue) chains of haptoglobi...

Structural studies on human type IV collagen.

Type IV collagen was isolated from limited pepsin digests of human placenta by selective salt precipitation at acidic and neutral pH. The native protein was resistant to human skin collagenase but was cleaved by a rat mast cell protease. Molecular sieve chromatography of the reduced and alkylated material separated relatively homogeneous components of molecular weight 140,000 (140K) and 100,000...

The determination of haptoglobins in normal human serum.

Studies on the prolactin-like substance in human placenta. IV.

In the previous papers (Ito and Higashi, 1961; Higashi, 1961), it was reported that a protein which stimulates the increase of the number and size of the fatty granules in the crop milk of pigeons was obtained from human placenta by the fractional precipitation with ammonium sulfate. The present communication describes the procedure of purification of this biologically active substance and some...

Haptoglobins in chronic lymphatic leukaemia.

Haptoglobin phenotypes have been determined in 30 chronic lymphatic leukaemia patients and concentrations determined in 21 of these. The number ofHpl phenotypes is slightly raised but not significantly so compared with a control population and is the same as that in first-degree relatives. Haptoglobin concentrations show higher concentrations in Hpl than Hp2, as has previously been reported. It...